Common questions

Why are suspension cell lines better than adherent cell lines?

Why are suspension cell lines better than adherent cell lines?

However, many cell lines can also be adapted for suspension culture….Characteristics of Each Culture Type.

Adherent Cell Culture Suspension Cell Culture
Growth is limited by surface area, which may limit product yields Growth is limited by concentration of cells in the medium, which allows easy scale-up

What is the purpose of cell suspension?

Cell Suspension is a type of bioink in which single cells or aggregates of cells multiply as they lie suspended in a predefined cell media. This bioink has been used in inkjet printers [54], and the cells can be used to aggregate without the need for a scaffold [55,56].

Can white blood cells grow in suspension?

There are cells growing in suspension, or in adherent conditions, which are reflected by the basic morphological and physiological characteristics of the cells. Lymphocytes grow in suspension, whereas cells such as keratinocytes, epithelial or endothelial cells grow as adherent cells.

How long can cells stay in suspension?

All Answers (2) Cell-types vary with respect to their response to suspension in PBS. Therefore, it is safe to say that your cells should be OK on ice in PBS for 15-20 min. Longer incubation more than 30 min may require frequent gentle mixing.

How long do cell lines last?

Cell lines can be finite or continuous. An immortalized or continuous cell line has acquired the ability to proliferate indefinitely, either through genetic mutations or artificial modifications. A finite cell line has been sub-cultured for 20-80 passages after which they senesce.

What is the meaning of cell suspension?

A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. The cells themselves can either be derived from homogenized tissue or from another type of culture.

How do you pass a suspension cell?

This can be done by directly diluting the cells in the culture flask and continue expanding them, or by withdrawing a portion of the cells from the culture flask and diluting the remaining cells down to a seeding density appropriate for the cell line.

How do you maintain suspension cells?

Passaging Suspension Cultures Replacement of growth medium is not carried out in suspension cultures; instead, the cells are maintained by feeding them every 2 to 3 days until they reach confluency.

How are suspension cells treated?


  1. Prepare a fresh solution of 4% paraformaldehyde in PBS.
  2. Wash the suspension cells twice with PBS by centrifugation at 200g for 5 minutes.
  3. Carefully resuspend the cell pellet in 4% paraformaldehyde in PBS at ~106 cells/ml.
  4. Wash the cells by centrifugation at 200g for 5 minutes and resuspend in PBS.

Can a cell line grow in suspension culture?

However, many cell lines can also be adapted for suspension culture. Similarly, most of the commercially available insect cell lines grow well in monolayer or suspension culture. Cells that are cultured in suspension can be maintained in culture flasks that are not tissue-culture treated,…

What is the difference between adherent and suspension cell lines?

Adherent cell lines are the cell lines, in which the primary cultures are attached to a solid support, and thus they are anchorage-dependent cells. Suspension cell lines are the cell lines in which the cultures are suspended in liquid media, and the cells thus remain in the fluid media.

What makes a suspension cell line anchorage independent?

Suspension cell lines are anchorage independent. They are derived from the suspension culture that can readily grow in a suspended liquid media. The suspension cell cultures should be continuously agitated to maintain its growth.

Why are suspension cells used in commercial production?

The growth of the suspension cells is limited by the concentration of the cells in the medium. Thus, over time the growth factors and media constituents deplete limiting the growth of the cells. Suspension cell lines are the most widely used type of primary cell lines in commercial production.