What is the function of RFC in eukaryotic?

What is the function of RFC in eukaryotic?

RFC is used in eukaryotic replication as a clamp loader, similar to the γ Complex in Escherichia coli. Its role as clamp loader involves catalysing the loading of PCNA onto DNA. It binds to the 3′ end of the DNA and uses ATP to open the ring of PCNA so that it can encircle the DNA.

What does replication factor C do?

Replication factor C (RFC) is a multisubunit complex that opens the sliding clamp and loads it onto the DNA chain in an ATP-dependent manner and is thus critical for high-speed DNA synthesis.

What is the function of replication factor C is eukaryotic replication?

Replication factor C (RFC) and proliferating cell nuclear antigen (PCNA) are accessory proteins essential for processive DNA synthesis in the domain Eucarya. The function of RFC is to load PCNA, a processivity factor of eukaryotic DNA polymerases δ and ɛ, onto primed DNA templates.

What does clamp loader do?

Clamp loaders are pentameric ATPases of the AAA+ family that operate to ensure processive DNA replication. They do so by loading onto DNA the ring-shaped sliding clamps that tether the polymerase to the DNA.

What is Primosome complex?

In molecular biology, a primosome is a protein complex responsible for creating RNA primers on single stranded DNA during DNA replication. The primosome consists of seven proteins: DnaG primase, DnaB helicase, DnaC helicase assistant, DnaT, PriA, Pri B, and PriC. This structure is referred to as the pre-primosome.

What is the role of PCNA in DNA replication?

Proliferating cell nuclear antigen (PCNA) plays an essential role in nucleic acid metabolism as a component of the replication and repair machinery. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis.

What is RPA in biology?

Replication protein A (RPA) is the major protein that binds to single-stranded DNA (ssDNA) in eukaryotic cells. During DNA replication, RPA prevents single-stranded DNA (ssDNA) from winding back on itself or from forming secondary structures.

What is the gamma complex?

The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta’:gamma(3):delta.

What is the function of the primase?

Primase functions by synthesizing short RNA sequences that are complementary to a single-stranded piece of DNA, which serves as its template. It is critical that primers are synthesized by primase before DNA replication can occur.