Common questions

What is serum media?

What is serum media?

Serum is vitally important as a source of growth and adhesion factors, hormones, lipids, and minerals for the culture of cells in basal media. In addition, serum also regulates cell membrane permeability and serves as a carrier for lipids, enzymes, micronutrients, and trace elements into the cell.

What are the 5 main components of cell culture base media?

A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors. In addition to nutrients, the medium also helps maintain pH and osmolality.

What is complete media cell culture?

Complete Cell Culture Medium is a complete medium designed for the culture of animal and human Cells. It was tested and optimized with Cell culture in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.

What constitutes the serum used for cell culture media?

Serum is a complex mix of albumins, growth factors and growth inhibitors and is probably one of the most important components of cell culture medium. The most commonly used serum is fetal bovine serum (FBS). Other types of serum are available including newborn calf serum and horse serum.

Why do we use serum-free media?

Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free as possible of confounding variables.

What are the main components of basal media?

Basal media contain amino acids, glucose, and ions (calcium, magnesium, potassium, sodium, and phosphate) essential for cell survival and growth.

How do you prepare a complete media?

To prepare complete, supplemented tissue culture media. This procedure is used to prepare 5% and 10% FBS supplemented media for tissue culture. Serum and additional supplements are added to tissue culture media prior to use. FBS must be heat inactivated before adding to media to eliminate complement activity.

What are the advantages of serum-free media?

The main advantages of using serum-free media are:

  • Increased definition and more reproducible formulations, allowing for more convincing and consistent research results.
  • Easier purification and downstream processing.
  • Lower risk of contamination with bacteria, fungi or virus.
  • Elimination of time-consuming batch tests.

What is the function of serum?

The human serum is a circulating carrier of exogenous and endogenous liquids in the blood. It allows substances to stick to the molecules within the serum and be buried within it. Human serum thus helps in the transportation of fatty acids and thyroid hormones which act on most of the cells found in the body.

Where is the GIBCO cell culture media formulation tool made?

Gibco cell culture products are manufactured in facilities that are compliant with current good manufacturing practices (GMP) for medical devices and adheres to a robust quality management system.

How is GIBCO used as a basal medium?

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, High Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications. Liver microsomes prepared from a pool of male Cynomologous Monkeys.

How much protein is in GIBCO cell culture medium?

Total protein concentration: 20mg/mL. Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement.

What does GIBCO stand for in cell biology?

Our Gibco extracellular matrices, scaffolds, and proteins provide in vivo –like morphology and physiologically relevant environments for more realistic cell biology and better intercellular interactions.