What is the Kirby-Bauer method?

The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic susceptibility test in determining what choice of antibiotics should be used when treating an infection. This method relies on the inhibition of bacterial growth measured under standard conditions.

What media is used for the Kirby-Bauer method?

The standard medium for the Kirby-Bauer method of susceptibility testing is Mueller-Hinton agar (MHA) [6].

What does the Kirby-Bauer test measure?

The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.

Why do we use Mueller-Hinton agar?

Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing, specifically disk diffusion tests. It is also used to isolate and maintain Neisseria and Moraxella species.

Is a large zone of inhibition good?

The size of the zone of inhibition is usually related to the level of antimicrobial activity present in the sample or product – a larger zone of inhibition usually means that the antimicrobial is more potent.

What do you need to know about the Kirby Bauer test?

Kirby-bauer test. antimicrobial-impregnated paper disks are placed on plate inoculated to form a bacterial lawn. the plates are incubated and time is given for agent to diffuse into agar. The resulting concentration gradient establishes the organisms supposed susceptibility to supposed agent.

How does the concentration gradient in Kirby Bauer work?

The resulting concentration gradient establishes the organisms supposed susceptibility to supposed agent. area of clearing around antimicrobial disks that depends upon the sensitivity of bacteria to the specific antimicrobial agent. results may kill the bacteria or simply prevent its growth.

Which is a qualitative variation of Kirby-Bauer disk diffusion test?

So, the definition of a quantitative or qualitative test is determined by the reporting method rather than the testing method. One variation of the Kirby-Bauer disk diffusion test is the E test (bioMérieux, Lyon, France), which is a gradient diffusion test.